Research of proliferation by valproic acid combined with cisplatin on human hepatoma cell

Objective To investigate the proliferation inhibition effect of valproic acid( VPA) combined with cisplatin( DDP) on human hepatoma cell HepG2,BEC7404 and SMMC7721. Methods The single use and combination use of three sets of concentration of VPA and DDP w ere given to human hepatoma cell HepG2,BEC7404,SMMC7721. After 24,48 and 72 h,the changes in the number and morphology of cell w as observed; and the cell proliferation inhibition rate( IR) of the drug w as analyzed by MTT assay; the cell proliferation inhibition rate q value of combination therapy w as calculated,and the synergistic effect w hether used in combination w as investigated. Results After72 h,the number of cells in each treatment group decreased obviously,and the decrease of VPA + DDP group w as more significant than that of single group,w ith great change in morphology. MTT assay show ed that the cell grow th rate of control group w as significantly higher than that of each experimental group; the VPA + DDP group had more significant effect on cell proliferation IR; w ith the increase in drug concentration and prolong duration of action,the proliferation IR of each group increased significantly. According to the q value in higher concentration of VPA( ≥ 100 μg / mL),VPA + DDP group had a significant synergistic effect. Conclusion VPA can enhance the proliferation of DDP on human hepatoma cell,and they have a synergistic advantage w hen VPA in a higher concentration.