Microcystin-LR induces the generation of oxygen free radical determined by different fluorescent probe

Objective To study the generation of superoxide and hydroxyl radical in human liver cells treated with Microcystin-LR(MCLR) determined by different fluorescent probe.Methods Human hepatoma cell lines HepG2 were treated with MCLR,then the generation of superoxide and hydroxyl radical was determined by the hydroethidine(HE) and 2-[6-(4'-hydroxy) phenoxy-3H-3-on-9-yl]benzoic acid(HPF).Results After exposure to 30 μmol/L and higher concentrations of MC-LR for 4 h,the generation of superoxide and hydroxyl radical in MC-LR-treated group was significantly higher than that of solvent control group(P0.05).The generation of superoxide and hydroxyl radical in the group that received combined treatment with deferoxamine(DFO) and MC-LR was significantly lower than that of 100 μ mol/L MC-LR-treated group(P0.05).Conclusion MCLR induces the generation of superoxide and hydroxyl radical in human hepatoma cell lines.Fluorescence probe HE and HPF is reliable and sensitive method for detecting the superoxide and hydroxyl radical.