Microviscosity of serum lipoproteins of different classes of mammals was estimated by eximerization of pyrene fluorescent probe. It has been shown that protein-lipid particles of high density have the highest microviscosity, while lipoproteins of low density--the lowest. For apoB-containing lipoproteins of different mammals a high positive correlation was found between pyrene fluorescence parameter, reflecting microviscosity and cholesterin-phospholipid ratio in the particles.