MTT assay applied to detect the toxicity of drug on fish cell lines

OD value in MTT assay had remarkable linear correlation with cell number,so it could well present the density of viable cells.Concentration and response time of MTT had great influence on that value,and the best of them were chosen to be 0.5 mg/mL and 4 h respectively according to the result in MTT test.Inoculating cell number had certain effect on the viability of cells.It was optimum that fish cell lines inoculated by 2.0×104 cells in each well(equal to 105 cell/mL) and the control SMMC7721 inoculated by 0.5×104 cells(equal to 2.5×104 cell/mL).Examined with the optimized MTT method and fit by the Logistic model,IC50 of various drugs to inhibit cell viability were acquired.IC50 values revealed that polyaromatic hydrocarbons had higher cytotoxicity than antibiotics.Furthermore,IC50 values of drugs in CIK,GCL and PCK cell lines were higher than those in SMMC7721(P0.05).