油红O
原代培养
细胞分化
细胞培养
体外
染色
生物
细胞生长
脂肪组织
脂肪生成
细胞生物学
内分泌学
分子生物学
化学
基因
生物化学
遗传学
作者
Yong Cai,Ayimuguli,Jutian Yang,Zufu Ma,LU Jian-xiong,Rongxin Zang,WU Jian-ping
出处
期刊:Chinese Journal of Animal Nutrition
日期:2010-01-01
卷期号:22 (6): 1768-1774
摘要
The study was conducted to set up a method for primary culture of ovine preadipocytes,and probe the mechanism of fat deposition in ruminants.Preadipocytes were isolated from perinephrit fat of 1-day-old ovine and cultured by digestive and explanting methods.The morphological changes of cultured cells were observed,growth curve was determined,intracellular fat contents were measured by the method of oil red O staining,and LPL,PPARγ and FAS expression levels were analyzed by real-time PCR.Results showed as follows: primary cultured cells showed homogeneous cell components,vigorous proliferation and high differentiation ratio and presented the morphology characteristics and idiotype genes of preadipocytes.All the results verified their preadipocyte identity.In this paper,a method for primary culture of preadipocytes was successfully set,and the processes of proliferation and differentiation were exhibited in vitro.
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