化学
吖啶橙
牛血清白蛋白
猝灭(荧光)
能量转移
荧光
接受者
血清白蛋白
费斯特共振能量转移
结合常数
吖啶
光化学
生物物理学
结合位点
色谱法
生物化学
有机化学
化学物理
生物
量子力学
物理
细胞凋亡
凝聚态物理
作者
Xinyu Li,Lin Zhang,Lin-Jin Yang,Chen Wang,Cuiping Bai
出处
期刊:Talanta
[Elsevier]
日期:1998-12-07
卷期号:47 (5): 1223-1229
被引量:359
标识
DOI:10.1016/s0039-9140(98)00198-2
摘要
The results from the measurement of the fluorescence spectrum showing the binding characteristics of acridine orange (AO) and bovine serum albumin (BSA) are reported. It was found that the equilibrium constant k=4848.64 l mol(-1), and the number of binding sites n=0.82. Based on the mechanism of the Forster energy transference, the transfer efficiency of energy and distance between the acceptor AO and BSA were found. The interaction between AO and BSA have been verified as consistent with the static quenching procedure and the quenching mechanism is related to the energy transfer.
科研通智能强力驱动
Strongly Powered by AbleSci AI