免疫系统
间质细胞
转录组
生物
乳腺癌
表型
癌症研究
细胞
肿瘤异质性
肿瘤微环境
癌症
单细胞分析
癌细胞
免疫学
基因表达
基因
遗传学
作者
Wingyan Chung,Hye Hyeon Eum,Hae Ock Lee,Kyung Min Lee,Han‐Byoel Lee,Kyu Tae Kim,Han Suk Ryu,Sang-Min Kim,Jeong Eon Lee,Yeon Hee Park,Zhengyan Kan,Wonshik Han,Woong-Yang Park
摘要
Abstract Single-cell transcriptome profiling of tumour tissue isolates allows the characterization of heterogeneous tumour cells along with neighbouring stromal and immune cells. Here we adopt this powerful approach to breast cancer and analyse 515 cells from 11 patients. Inferred copy number variations from the single-cell RNA-seq data separate carcinoma cells from non-cancer cells. At a single-cell resolution, carcinoma cells display common signatures within the tumour as well as intratumoral heterogeneity regarding breast cancer subtype and crucial cancer-related pathways. Most of the non-cancer cells are immune cells, with three distinct clusters of T lymphocytes, B lymphocytes and macrophages. T lymphocytes and macrophages both display immunosuppressive characteristics: T cells with a regulatory or an exhausted phenotype and macrophages with an M2 phenotype. These results illustrate that the breast cancer transcriptome has a wide range of intratumoral heterogeneity, which is shaped by the tumour cells and immune cells in the surrounding microenvironment.
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