生物
肌动蛋白
细胞生物学
神经元肌动蛋白重塑
基因亚型
肌动蛋白重塑
病毒
MDia1公司
细胞质
共域化
牛痘
肌动蛋白细胞骨架
细胞骨架
病毒学
细胞
生物化学
基因
重组DNA
作者
N. Bishara Marzook,Sharissa L. Latham,Helena Lynn,Christopher McKenzie,Christine Chaponnier,Georges E. Grau,Timothy P. Newsome
出处
期刊:Cytoskeleton
[Wiley]
日期:2017-03-17
卷期号:74 (4): 170-183
被引量:8
摘要
Actin is a major component of the cytoskeleton and is present as two isoforms in non-muscle cells: β- and γ-cytoplasmic actin. These isoforms are strikingly conserved, differing by only four N-terminal amino acids. During spread from infected cells, vaccinia virus (VACV) particles induce localized actin nucleation that propel virus to surrounding cells and facilitate cell-to-cell spread of infection. Here we show that virus-tipped actin comets are composed of β- and γ-actin. We employed isoform-specific siRNA knockdown to examine the role of the two isoforms in VACV-induced actin comets. Despite the high level of similarity between the actin isoforms, and their colocalization, VACV-induced actin nucleation was dependent exclusively on β-actin. Knockdown of β-actin led to a reduction in the release of virus from infected cells, a phenotype dependent on virus-induced Arp2/3 complex activity. We suggest that local concentrations of actin isoforms may regulate the activity of cellular actin nucleator complexes.
科研通智能强力驱动
Strongly Powered by AbleSci AI