Isolation, Characterization and Growth-Promoting Properties of Phosphate-Solubilizing Bacteria (PSBs) Derived from Peach Tree Rhizosphere

Microbial fertilizers have a significant role in promoting plant growth, resistance to environmental stresses, and soil remediation. Microbial fertilizers are mainly composed of beneficial microorganisms that contain specific functions. Focusing on the peach tree rhizosphere region, this study aimed to isolate and screen bacteria with efficient phosphate-solubilizing capacity for application in microbial fertilizers, as well as to dig deeper into the other properties of the strains to further explore the roles of these phosphate-solubilizing bacteria (PSBs) in terms of plant growth in order to provide valuable microbial resources for microbial fertilizer development. By collecting soil samples from peach tree rhizospheres, we initially screened 86 PSB strains using the plate method and determined the phosphate-solubilizing capacity (ranged from 0 to 14 μg/mL). Afterwards, 51 strains with strong phosphate-solubilizing capacity were selected for molecular identification; the strains belonged to 12 genera, with Bacillus and Burkholderia accounting for the majority. Concurrent evaluation of iron carriers and indoleacetic-3-acid (IAA) production capabilities identified strain WPD85 as exhibiting dual functionality with strong performance in both parameters. Subsequently, we combined the analysis of phosphate-solubilizing capacity and growth-promoting properties to select eight strains of PSBs; characterized them physiologically, biochemically, and molecularly; determined the biofilm-forming capacity; and conducted potting experiments. Notably, strain WPD103 exhibited exceptional biofilm-forming capacity (OD595 = 1.09). Of particular interest, strain WPD16 demonstrated both an elevated inorganic phosphate solubilization index (D/d = 2.99) and remarkable iron carriers production capacity, while peach seedlings treated with WPD16 exhibited 119% enhancement in plant height increment compared to the control. This study enhances our understanding of PSB traits and identifies Burkholderia sp. WPD16 as a strategic candidate for developing targeted microbial fertilizers, offering a sustainable solution to reduce reliance on chemical inputs in orchard management.