化学
系统药理学
中医药
计算生物学
机制(生物学)
强直性脊柱炎
药理学
医学
交互网络
生物信息学
传统医学
药物发现
化学
生物
药品
生物化学
内科学
替代医学
哲学
认识论
病理
基因
作者
Zhenyu Zheng,Yidi Wang,Malong Guo,Jiayi Guo,Jingang Cui,Wenxiao Zhu,Weidong Chen,Yonghui Liu,Hongxun Cui
标识
DOI:10.1016/j.compbiomed.2023.107206
摘要
Some Traditional Chinese Medicine (TCM) has shown anti-inflammatory and immunosuppressive effects on Ankylosing Spondylitis (AS) treatment. Wan Bikang (WBK) and Wan Biqing (WBQ) are two traditional empirical formulas for AS. However, the mechanism of their effects on AS is largely unknown. This study deciphered the underlying common molecular mechanisms of these TCM treatments for AS. The ultra-high-performance liquid chromatography-triple/time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) assays were employed to detect herbal ingredients. Target proteins of herbal ingredients were identified by ChEMBL Database. To infer the relationships between ingredients and AS-related proteins, network pharmacology was employed. Protein-protein interaction (PPI) network and core target analyses were carried out with tools Cytoscape and STRING. To find out the molecular basis and target of AS, molecular docking and an in vitro experiment were also conducted. It is found that estradiol may participate in the treatment of AS via the inhibition of inflammatory factors, and Estrogen Receptor 1 (ESR1) appears to be a key target. This research offers insight into the therapeutic mechanism of TCM formulas for AS and furthers our understanding of TCM pharmacology.
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