微尺度化学
微流控
数字微流体
纳米技术
化学发光
计算机科学
自动化
电润湿
工艺工程
工程类
材料科学
电气工程
电介质
化学
机械工程
数学教育
有机化学
数学
作者
Kaixin Su,Jiaqi Li,Hailan Liu,Yuan Zou
出处
期刊:Micromachines
[Multidisciplinary Digital Publishing Institute]
日期:2024-11-08
卷期号:15 (11): 1358-1358
被引量:2
摘要
Technologies based on digital microfluidics (DMF) have made significant advancements in the automated manipulation of microscale liquids and complex multistep processes. Due to their numerous benefits, such as automation, speed, cost-effectiveness, and minimal sample volume requirements, these systems are particularly well suited for immunoassays. In this review, an overview is provided of diverse DMF manipulation platforms and their applications in immunological analysis. Initially, droplet-driven DMF platforms based on electrowetting on dielectric (EWOD), magnetic manipulation, surface acoustic wave (SAW), and other related technologies are briefly introduced. The preparation of DMF is then described, including material selection, fabrication techniques and droplet generation. Subsequently, a comprehensive account of advancements in the integration of DMF with various immunoassay techniques is offered, encompassing colorimetric, direct chemiluminescence, enzymatic chemiluminescence, electrosensory, and other immunoassays. Ultimately, the potential challenges and future perspectives in this burgeoning field are delved into.
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