伤口愈合
芒果藤黄
化学
皮肤修复
生物医学工程
药理学
色谱法
传统医学
医学
外科
作者
Rathapon Asasutjarit,Chawisa Leenabanchong,Sewan Theeramunkong,Adryan Fritiohady,Thunyathorn Yimsoo,Witchuda Payuhakrit,Udomluk Sukatta,Asira Fuongfuchat
标识
DOI:10.1016/j.ijpharm.2023.122812
摘要
Xanthones (XTs) are bioactive compounds found in mangosteen trees (Garcinia mangostana Linn.). They are used as an active ingredient in various health products. However, there is a lack of data of their application in wound healing. In particular, the topical products of XTs for wound healing; they should be sterilized to minimize the risks of wound infection from contaminated microorganisms. This study thus aimed to optimize the formulation of sterilized XTs-loaded nanoemulgel (XTs-NE-G) and to investigate their wound healing activities. The XTs-NE-Gs were prepared by mixing various gels containing sodium alginate (Alg) and Pluronic F127 (F127) into a XTs-nanoemulsion (NE) concentrate according to the face-centered central composite design. The results showed that the optimized XTs-NE-G was A5-F3 containing 5% w/w Alg and 3% w/w F127. It enhanced the proliferation-, migration rates of skin fibroblasts (HFF-1 cells) with an optimal viscosity. After blending the XTs-NE concentrate and the gel that was previously sterilized by a membrane filtration and an autoclaving technique, respectively, the sterilized A5-F3 was obtained. The sterilized A5-F3 still had effective bioactivities towards the HFF-1 cells. It promoted re-epithelialization, collagen deposition and inflammation suppression in the mice’ wounds. It could thus be accepted for further investigation in clinical studies.
科研通智能强力驱动
Strongly Powered by AbleSci AI