清脆的
Cas9
计算生物学
蓝图
生物
基因组编辑
回文
效应器
合成生物学
计算机科学
遗传学
基因
工程类
细胞生物学
机械工程
作者
Jack P. K. Bravo,Grace Hibshman,David W. Taylor
标识
DOI:10.1016/j.copbio.2022.102839
摘要
Clustered regularly interspaced short palindromic repeats - CRISPR-associated protein (CRISPR-Cas) systems are a critical component of the bacterial adaptive immune response. Since the discovery that they can be reengineered as programmable RNA-guided nucleases, there has been significant interest in using these systems to perform diverse and precise genetic manipulations. Here, we outline recent advances in the mechanistic understanding of CRISPR-Cas9, how these findings have been leveraged in the rational redesign of Cas9 variants with altered activities, and how these novel tools can be exploited for biotechnology and therapeutics. We also discuss the potential of the ubiquitous, yet often-overlooked, multisubunit CRISPR effector complexes for large-scale genomic deletions. Furthermore, we highlight how future structural studies will bolster these technologies.
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