Licorice Extract Alleviates Intestinal Inflammation Caused by Lipopolysaccharide (Lps) in Loach (Misgurnus Anguillicaudatus)

Some chemicals from licorice such as glycyrrhizic acid, liquiritigenin, liquiritin and glycyrrhizin polysaccharide make an momentous role in anti-inflammatory, immune regulation, intestinal microflora regulation, cell apoptosis regulation and oxidative stress reduction. Therefore, in order to study the therapeutic influence of licorice extract on inflammation of loach, we inject lipopolysaccharide (LPS) into loach to induced intestinal inflammation. We randomly divided the loaches into two groups, one group fed with loach feed mixed with licorice extract and the other fed with ordinary loach feed as control. After 24 hours of experiment, liver of loach was collected for transcriptome sequencing to evaluate the effect of licorice extract on inflammation. Transcriptome analysis showed that there were 9246 DEGs, with 5241 up-regulated and 4005 down-regulated expression. Combining the analysis of GO and KEGG enrichment, it is speculated that licorice treatment can up-regulate the expression of Claudin in tight junction proteins caused by LPS treatment to improve intestinal mucosal damage and reach therapeutic effect. Regulation of actin cytoskeleton may has great impact on the regulation of inflammation. The enrichment of metabolic pathways may not only be caused by metabolic disorders, but also related to the regulation of inflammation. This research provides a molecular mechanism for elucidating the therapeutic efficacy of licorice extract on intestinal inflammation of loaches.