Covalent organic framework in-situ immobilized laccase for the covalent polymerization removal of sulfamethoxazole in the presence of natural phenols: Prominent enzyme stability and activity

In current water treatment processes, pollutants are typically degraded into small molecules and CO2 for detoxification. This study employed laccase-mediated aggregation of new pollutants with natural phenolic compounds to remove pollutants by forming large molecular substances, effectively sequestering carbon. Free laccase is susceptible to environmental influences, causing deactivation. However, immobilizing laccase onto a carrier enhances enzyme stability. In the experiment, laccase was immobilized onto the covalent organic framework TpPa-1 through an in-situ loading process, resulting in immobilized laccase Lac@TpPa-1. Stability studies revealed that immobilized laccase outperformed free laccase in terms of pH, temperature, and recyclability. Moreover, immobilized laccase was employed for catalyzing the removal of emerging pollutants containing natural phenolic compounds, achieving an 80.53% removal rate with the addition of 0.02 g of laccase within 5 h. Analytical techniques like Fourier-transform ion cyclotron resonance mass spectrometry were used to uncover reaction pathways, demonstrating the presence of radical polymerization and 1, 4 nucleophilic addition. This research utilized TpPa-1 as a carrier for laccase immobilization, promoting oxidation-induced polymerization for efficient pollutant removal. It provides a theoretical foundation for understanding the interplay between emerging pollutants and phenolic compounds in natural environments and enhances the practical application of laccase through immobilization.