SPMD公司
纳米孔
纳米载体
材料科学
纳米技术
检出限
纳米结构
纳米传感器
DNA
生物物理学
化学
计算机科学
生物
色谱法
药物输送
生物化学
操作系统
作者
Xian Zhang,Dan Luo,Yi Zheng,Xiao-Qiong Li,Juan Song,Weiwei Zhao,Hong‐Yuan Chen,Jing‐Juan Xu
出处
期刊:ACS Nano
[American Chemical Society]
日期:2022-09-01
卷期号:16 (9): 15108-15114
被引量:10
标识
DOI:10.1021/acsnano.2c06303
摘要
The use of functional DNA nanostructures as carriers to ship proteins through solid-state nanopores has recently seen substantial growth in single-protein-molecule detection (SPMD), driven by the potential of this methodology and implementations that it may enable. Ultrasmall nanopores have exhibited obvious advantages in spatiotemporal biological detection due to the appropriate nanoconfined spaces and unique properties. Herein, a 6.8 nm DNA tetrahedron (TDN) with a target-specific DNA aptamer (TDN-apt) was engineered to carry the representative target of acetylcholinesterase (AChE) through an ultrasmall nanopipet with a 30 nm orifice, underpinning the advanced SPMD of AChE with good performance in terms of high selectivity, low detection limit (0.1 fM), and especially superior signal-to-noise ratio (SNR). The kinetic interaction between TDN-apt and AChE was studied and the practical applicability of the as-developed SPMD toward real samples was validated using serum samples from patients with Alzheimer's disease. This work not only presented a feasible SPMD solution toward low-abundance proteins in complex samples and but also was envisioned to inspire more interest in the design and implementation of synergized DNA nanostructure-ultrasmall nanopore systems for future SPMD development.
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