腐败舍瓦内拉菌
尸体
铁载体
腐胺
化学
鸟氨酸脱羧酶
胍丁胺
生物化学
乙二胺
立体化学
酶
细菌
生物
有机化学
基因
遗传学
作者
Cho Zin Soe,Amalie A. H. Pakchung,Rachel Codd
标识
DOI:10.1002/cbdv.201200014
摘要
Abstract To manage iron acquisition in an oxic environment, Shewanella putrefaciens produces the macrocyclic dihydroxamic acid putrebactin (PB) as its native siderophore. In this work, we have established the siderophore profile of S. putrefaciens in cultures augmented with the native PB precursor putrescine and in putrescine‐depleted cultures. Compared to base medium, PB increased by two‐fold in cultures of S. putrefaciens with 10 m M NaCl and 20 m M exogenous putrescine. In cultures augmented with 1,4‐diaminobutan‐2‐one (DAB), PB decreased with only 0.02‐fold PB detectable at 10 m M DAB. As an ornithine decarboxylase (ODC) inhibitor, DAB depleted levels of endogenous putrescine which attenuated downstream PB assembly. Under putrescine‐depleted conditions, S. putrefaciens produced as its replacement siderophore the cadaverine‐based desferrioxamine B (DFO‐B), as characterised by ESI‐MS of the Fe III ‐loaded form ( m / z obs 614.13; m / z calc 614.27). A third siderophore, independent of DAB, was observed in low levels. LC/MS Analysis of the Fe III ‐loaded extract gave m / z obs 440.93, which, formulated as a 1 : 1 Fe III complex with a macrocyclic dihydroxamic acid, comprising one putrescine‐ and one cadaverine‐based precursor ( m / z calc 440.14). These results show that the production of native PB or non‐native DFO‐B by S. putrefaciens can be directed though upstream inhibition of ODC. This approach could be used to increase the molecular diversity of siderophores produced by S. putrefaciens and to map alternative diamine‐dependent metabolites.
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