Bioenergy Beads: A Tool for Regeneration of ATP/NTP in Biocatalytic Synthesis

Active inclusion bodies of recombinant polyphosphate kinase were obtained by simple washing of Escherichia coli cells with nonionic detergent and then they were immobilized in agar/TiO2 beads. Bioenergy beads obtained are charged by polyphosphate to act as rechargeable suppply of adenosine/nucleoside triphosphates (ATP/NTP), a practical tool for synthesis of artificial receptors.