TRPV1型
化学
兴奋剂
感受野
全身给药
药理学
背根神经节
瞬时受体电位通道
钠通道
神经病理性疼痛
伤害
内分泌学
钠通道阻滞剂
内科学
脂毒素
麻醉
运动前神经元活动
中枢神经系统
脊髓
医学
受体
钠
神经科学
生物
体内
有机化学
生物技术
精神科
作者
Steve McGaraughty,Katharine L. Chu,Marc J. C. Scanio,Michael E. Kort,Connie R. Faltynek,Michael F. Jarvis
标识
DOI:10.1124/jpet.107.134148
摘要
We have recently reported that systemic delivery of A-803467 [5-(4-chlorophenyl-N-(3,5-dimethoxyphenyl)furan-2-carboxamide], a selective Nav1.8 sodium channel blocker, reduces behavioral measures of chronic pain. In the current study, the effects of A-803467 on evoked and spontaneous firing of wide dynamic range (WDR) neurons were measured in uninjured and rats with spinal nerve ligations (SNLs). Administration of A-803467 (10–30 mg/kg i.v.) reduced mechanically evoked (10-g von Frey hair) and spontaneous WDR neuronal activity in SNL rats. In uninjured rats, A-803467 (20 mg/kg i.v.) transiently reduced evoked but not spontaneous firing of WDR neurons. The systemic effects of A-803467 in SNL rats were not altered by spinal transection or by systemic pretreatment with the transient receptor potential vanilloid type 1 (TRPV1) receptor agonist, resiniferatoxin, at doses that impair the function of TRPV1-expressing fibers. To determine sites of action, A-803467 was administered into spinal tissue, into the uninjured L4 dorsal root ganglion (DRG), or into the neuronal receptive field. Injections of A-803467 into the L4 DRG (30–100 nmol/1 μl) or into the hindpaw receptive field (300 nmol/50 μl) reduced evoked but not spontaneous WDR firing. In contrast, intraspinal (50–150 nmol/0.5 μl) injection of A-803467 decreased both evoked and spontaneous discharges of WDR neurons. Thus, Nav1.8 sodium channels on the cell bodies/axons within the L4 DRG as well as on peripheral and central terminals of primary afferent neurons regulate the inflow of low-intensity mechanical signals to spinal WDR neurons. However, Nav1.8 sodium channels on central terminals seem to be key to the modulation of spontaneous firing in SNL rats.
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