Nanoparticle PCR: Nanogold‐Assisted PCR with Enhanced Specificity

Angewandte Chemie International EditionVolume 44, Issue 32 p. 5100-5103 Communication Nanoparticle PCR: Nanogold-Assisted PCR with Enhanced Specificity† Haikuo Li, Haikuo Li Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361Search for more papers by this authorJiehuan Huang, Jiehuan Huang Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361Search for more papers by this authorJunhong Lv Dr., Junhong Lv Dr. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China, Fax: (+86) 21-5955-6902Search for more papers by this authorHongjie An, Hongjie An Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361Search for more papers by this authorXiaodong Zhang Dr., Xiaodong Zhang Dr. Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361Search for more papers by this authorZhizhou Zhang Prof. Dr., Zhizhou Zhang Prof. Dr. [email protected] Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361Search for more papers by this authorChunhai Fan Prof. Dr., Chunhai Fan Prof. Dr. [email protected] Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China, Fax: (+86) 21-5955-6902Search for more papers by this authorJun Hu Prof. Dr., Jun Hu Prof. Dr. [email protected] Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China, Fax: (+86) 21-5955-6902Search for more papers by this author Haikuo Li, Haikuo Li Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361Search for more papers by this authorJiehuan Huang, Jiehuan Huang Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361Search for more papers by this authorJunhong Lv Dr., Junhong Lv Dr. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China, Fax: (+86) 21-5955-6902Search for more papers by this authorHongjie An, Hongjie An Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361Search for more papers by this authorXiaodong Zhang Dr., Xiaodong Zhang Dr. Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361Search for more papers by this authorZhizhou Zhang Prof. Dr., Zhizhou Zhang Prof. Dr. [email protected] Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361Search for more papers by this authorChunhai Fan Prof. Dr., Chunhai Fan Prof. Dr. [email protected] Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China, Fax: (+86) 21-5955-6902Search for more papers by this authorJun Hu Prof. Dr., Jun Hu Prof. Dr. [email protected] Bio-X DNA Computer Consortium, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030, China, Fax: (+86) 21-6407-7361 Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China, Fax: (+86) 21-5955-6902Search for more papers by this author First published: 08 August 2005 https://doi.org/10.1002/anie.200500403Citations: 222 † This work was supported by the Chinese Academy of Sciences, the National Natural Science Foundation (10335070 and 20404016), the Ministry of Science and Technology of China (2002CCA00600, 2003A310A02, and 2003AA226011), the Shanghai Municipal Commission for Science and Technology (0452M068, 03DZ14025, and 02JC14029), and the Ministry of Personnel. We also thank Prof. Kevin Plaxco (UCSB) for helpful comments. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Graphical Abstract Nanogold may not glitter, but its presence significantly improves the specificity and yield of PCR reactions owing to the greater affinity of gold nanoparticles to single-stranded DNA than to double-stranded DNA which helps to reduce mispairing. Indeed, gel electrophoresis shows a single predominant band for the target DNA obtained by this method, in contrast to streaking bands for products obtained by conventional PCR (lanes 1 and 2; see image). Citing Literature Volume44, Issue32August 12, 2005Pages 5100-5103 RelatedInformation