检出限
免疫球蛋白E
软件可移植性
色谱法
流体学
化学
抗体
免疫学
计算机科学
医学
工程类
航空航天工程
程序设计语言
作者
T. Ohashi,Kazuma Mawatari,Kae Sato,Manabu Tokeshi,Takehiko Kitamori
出处
期刊:Lab on a Chip
[The Royal Society of Chemistry]
日期:2009-01-01
卷期号:9 (7): 991-991
被引量:83
摘要
We have developed a novel, practical micro-ELISA system for sensitive and rapid allergy diagnosis. The enzymatic reactions occurred under stopped-flow conditions, resulting in both high precision and high sensitivity. A BSA-biotin-avidin linker was introduced for the immobilization of water-soluble allergens on polystyrene microbeads, enabling immobilization of allergens in sufficient density to provide high sensitivity. Evaluation of the system's performance showed a good detection limit (2 ng/mL) for total IgE measurement. In addition, a good correlation with a conventional method (CAP method) was demonstrated using human serum samples from 85 allergy patients. Importantly, sample volumes (5 µL) were 10 times smaller and analysis time (12 min) was >20 times faster than the conventional method. All procedures were automatically regulated with our simple microfluidic system, and all the fluidic, optic and electronic components were integrated for portability. We believe that our system has the potential to become a very powerful tool, particularly for point-of-care diagnosis.
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