Development of Sensitive Immunoassays for Free and Total Human Glandular Kallikrein 2

前列腺癌 前列腺特异性抗原 抗体 前列腺 单克隆抗体 抗原 检出限 医学 内科学 男科 癌症 化学 泌尿科 免疫学 色谱法
作者
Ville Väisänen,Susann Eriksson,Kaisa K. Ivaska,Hans Lilja,Martti Nurmi,Kim Pettersson
出处
期刊:Clinical Chemistry [American Association for Clinical Chemistry]
卷期号:50 (9): 1607-1617 被引量:49
标识
DOI:10.1373/clinchem.2004.035253
摘要

Abstract Background: Free and total human kallikrein 2 (hK2) might improve the discrimination between prostate cancer and benign prostatic hyperplasia. Concentrations of hK2 are 100-fold lower than concentrations of prostate-specific antigen (PSA); therefore, an hK2 assay must have a low detection limit and good specificity. Methods: PSA- and hK2-specific monoclonal antibodies were used in solid-phase, two-site immunofluorometric assays to detect free and total hK2. The total hK2 assay used PSA-specific antibodies to block nonspecific signal. The capture antibody of the free hK2 assay did not cross-react with PSA. To determine the hK2 concentrations in the male bloodstream, total hK2 was measured in a control group consisting of 426 noncharacterized serum samples. Free and total hK2 were measured in plasma from 103 patients with confirmed prostate cancer. Results: All 426 males in the control group had a total hK2 concentration above the detection limit of 0.0008 μg/L. The median total hK2 concentration was 0.022 μg/L (range, 0.0015–0.37 μg/L). hK2 concentrations were 0.1–58% of total PSA (median, 3.6%). hK2 concentrations were similar in men 41–50 and 51–60 years of age. The ratio of hK2 to PSA steadily decreased from 5–30% at PSA <1 μg/L to 1–2% at higher PSA concentrations. In 103 patients with prostate cancer, the median hK2 concentration in plasma was 0.079 μg/L (range, 0.0015–16.2 μg/L). The median free hK2 concentration was 0.070 (range, 0.005–12.2) μg/L. The proportion of free to total hK2 varied from 17% to 131% (mean, 85%). Conclusions: The wide variation in the free-to-total hK2 ratio suggests that hK2 in blood plasma is not consistently in the free, noncomplexed form in patients with prostate cancer. The new assay is sufficiently sensitive to be used to study the diagnostic accuracies of free and total hK2 for prostate cancer.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
玩命的化蛹完成签到,获得积分10
1秒前
李健的小迷弟应助水杨酸采纳,获得10
2秒前
艺阳发布了新的文献求助10
2秒前
3秒前
3秒前
4秒前
CAOHOU应助康园采纳,获得10
4秒前
郭1994发布了新的文献求助10
4秒前
111发布了新的文献求助10
5秒前
7秒前
酷波er应助wrong采纳,获得10
7秒前
jiajiajai完成签到,获得积分10
7秒前
8秒前
蒲云海发布了新的文献求助100
8秒前
一碗冷的粥发布了新的文献求助100
10秒前
11秒前
11秒前
Lucas应助自由的馒头采纳,获得10
11秒前
12秒前
xzy998应助科研通管家采纳,获得10
12秒前
赘婿应助科研通管家采纳,获得10
12秒前
柯亦云应助科研通管家采纳,获得20
12秒前
林德洵应助科研通管家采纳,获得20
12秒前
鸣笛应助科研通管家采纳,获得100
12秒前
丘比特应助科研通管家采纳,获得10
13秒前
13秒前
柯亦云应助科研通管家采纳,获得30
13秒前
科研通AI2S应助科研通管家采纳,获得10
13秒前
淘朕朕完成签到,获得积分10
13秒前
认真三问发布了新的文献求助10
15秒前
bkagyin应助光亮君浩采纳,获得10
16秒前
LJX发布了新的文献求助10
16秒前
chengya发布了新的文献求助10
16秒前
十三完成签到 ,获得积分10
18秒前
亦安完成签到,获得积分10
19秒前
CAOHOU应助康园采纳,获得10
20秒前
张祖成完成签到,获得积分10
22秒前
23秒前
情怀应助光亮君浩采纳,获得10
24秒前
北彧发布了新的文献求助20
24秒前
高分求助中
【请各位用户详细阅读此贴后再求助】科研通的精品贴汇总(请勿应助) 10000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
International Code of Nomenclature for algae, fungi, and plants (Madrid Code) (Regnum Vegetabile) 500
Maritime Applications of Prolonged Casualty Care: Drowning and Hypothermia on an Amphibious Warship 500
Comparison analysis of Apple face ID in iPad Pro 13” with first use of metasurfaces for diffraction vs. iPhone 16 Pro 500
Towards a $2B optical metasurfaces opportunity by 2029: a cornerstone for augmented reality, an incremental innovation for imaging (YINTR24441) 500
Materials for Green Hydrogen Production 2026-2036: Technologies, Players, Forecasts 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4056230
求助须知:如何正确求助?哪些是违规求助? 3594329
关于积分的说明 11419977
捐赠科研通 3320180
什么是DOI,文献DOI怎么找? 1825613
邀请新用户注册赠送积分活动 896656
科研通“疑难数据库(出版商)”最低求助积分说明 817971