8‐Vinyl‐2′‐deoxyguanosine as a Fluorescent 2′‐Deoxyguanosine Mimic for Investigating DNA Hybridization and Topology

A one light strand: 2′-Deoxyguanosine was made fluorescent by attaching a vinyl group at the C8 position (see structure). The resulting fluorophore is highly sensitive towards DNA double-strand formation and alterations in the secondary structure of its parent oligonucleotide, such as formation of different quadruplex structures. These differences can be readily detected in the emission spectrum (see picture).