Hydrogel surfaces to promote attachment and spreading of endothelial progenitor cells

Journal of Tissue Engineering and Regenerative MedicineVolume 7, Issue 5 p. 337-347 Research Article Hydrogel surfaces to promote attachment and spreading of endothelial progenitor cells Gulden Camci-Unal, Gulden Camci-Unal Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USASearch for more papers by this authorJason William Nichol, Jason William Nichol Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USASearch for more papers by this authorHojae Bae, Hojae Bae Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USASearch for more papers by this authorHalil Tekin, Halil Tekin Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USASearch for more papers by this authorJoyce Bischoff, Joyce Bischoff Vascular Biology Program and Department of Surgery, Children's Hospital, Boston, MA, USA Department of Surgery, Harvard Medical School, Boston, MA, USASearch for more papers by this authorAli Khademhosseini, Corresponding Author Ali Khademhosseini Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USACorrespondence to: A. Khademhosseini, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA. E-mail: [email protected]Search for more papers by this author Gulden Camci-Unal, Gulden Camci-Unal Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USASearch for more papers by this authorJason William Nichol, Jason William Nichol Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USASearch for more papers by this authorHojae Bae, Hojae Bae Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USASearch for more papers by this authorHalil Tekin, Halil Tekin Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USASearch for more papers by this authorJoyce Bischoff, Joyce Bischoff Vascular Biology Program and Department of Surgery, Children's Hospital, Boston, MA, USA Department of Surgery, Harvard Medical School, Boston, MA, USASearch for more papers by this authorAli Khademhosseini, Corresponding Author Ali Khademhosseini Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USACorrespondence to: A. Khademhosseini, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA. E-mail: [email protected]Search for more papers by this author First published: 06 January 2012 https://doi.org/10.1002/term.517Citations: 58Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Endothelialization of artificial vascular grafts is a challenging process in cardiovascular tissue engineering. Functionalized biomaterials could be promising candidates to promote endothelialization in repair of cardiovascular injuries. The purpose of this study was to synthesize hyaluronic acid (HA) and heparin-based hydrogels that could promote adhesion and spreading of endothelial progenitor cells (EPCs). We report that the addition of heparin into HA-based hydrogels provides an attractive surface for EPCs promoting spreading and the formation of an endothelial monolayer on the hydrogel surface. To increase EPC adhesion and spreading, we covalently immobilized CD34 antibody (Ab) on HA–heparin hydrogels, using standard EDC/NHS amine-coupling strategies. We found that EPC adhesion and spreading on CD34 Ab-immobilized HA–heparin hydrogels was significantly higher than their non-modified analogues. Once adhered, EPCs spread and formed an endothelial layer on both non-modified and CD34 Ab-modified HA–heparin hydrogels after 3 days of culture. We did not observe significant adhesion and spreading when heparin was not included in the control hydrogels. In addition to EPCs, we also used human umbilical cord vein endothelial cells (HUVECs), which adhered and spread on HA–heparin hydrogels. Macrophages exhibited significantly less adhesion compared to EPCs on the same hydrogels. This composite material could possibly be used to develop surface coatings for artificial cardiovascular implants, due to its specificity for EPC and endothelial cells on an otherwise non-thrombogenic surface. Copyright © 2012 John Wiley & Sons, Ltd. Supporting Information Supporting information on the internet Filename Description term_517_figS1.tifTIFF image, 484.1 KB Figure S1. Flow cytometry analysis of EPCs and macrophages for the amount of CD34 expression term_517_figS2.tifTIFF image, 1.1 MB Figure S2. Adsorption of 1 mg/ml fluorescein–BSA on HA-based hydrogels term_517_figS3.tifTIFF image, 855.6 KB Figure S3. Effect of serum concentration in media on EPC attachment on 1% HAMA–2% HepMA hydrogels term_517_figS4.tifTIFF image, 611 KB Figure S4. 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