非结核分枝杆菌
套式聚合酶链反应
肺结核
染色
结核分枝杆菌复合物
结核分枝杆菌
分枝杆菌
耐酸
聚合酶链反应
生物
病理
肺
实时聚合酶链反应
医学
痰
内科学
基因
生物化学
作者
Yo Na Kim,Kyoung Min Kim,Ha Na Choi,Ju Hyun Lee,Ho Sung Park,Kyu Yun Jang,Woo Sung Moon,Myoung Jae Kang,Dong Geun Lee,Myoung Ja Chung
标识
DOI:10.1016/j.jmoldx.2015.04.005
摘要
The need for isolation of nontuberculous mycobacteria (NTM) from clinical specimens has increased in recent years. Our aim was to determine the clinical usefulness of PCR for differential diagnosis of tuberculosis and nontuberculous mycobacterial infection in lung tissue that show chronic granulomatous inflammation. A total of 199 formalin-fixed, paraffin-embedded specimens, including 137 Mycobacterium tuberculosis (MTB), 17 NTM cases, and 45 other than mycobacterial cases were collected. We performed acid-fast staining, MTB and NTM nested PCRs, and MTB and NTM real-time PCRs. No histologic difference between MTB and NTM infections was observed. Sensitivity and specificity for detecting MTB were 70.1% and 95.1% by nested PCR, respectively, and 70.8% and 100.0% by real-time PCR, respectively. Sensitivity and specificity for detecting NTM were 52.9% and 96.15% by nested PCR, respectively, and 35.3% and 100.0% by real-time PCR, respectively. Mycobacteria were identified by acid-fast staining in 50 of 154 cases (32.5%). All 50 acid-fast staining-positive cases showed positive nested and real-time PCR results (n = 47 MTB PCR positive; n = 3 NTM PCR positive), and results agreed with final diagnosis. PCR will be useful for the rapid diagnosis of mycobacterial infection and differentiation of MTB from NTM in formalin-fixed, paraffin-embedded specimens, especially in acid-fast staining-positive specimens.
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