焦点粘着
机械敏感通道
微管
串扰
细胞生物学
机械转化
细胞粘附
肌动蛋白
整合素
罗亚
乙酰化
细胞骨架
化学
生物物理学
细胞
生物
活体细胞成像
信号转导
离子通道
生物化学
物理
光学
基因
受体
作者
Shailaja Seetharaman,Benoît Vianay,Vanessa Roca,Chiara De Pascalis,Batiste Boëda,Florent Dingli,Damarys Loew,Stéphane Vassilopoulos,Manuel Théry,Sandrine Etienne-Manneville
标识
DOI:10.1101/2020.07.22.205203
摘要
Abstract Mechanotransduction is a process by which cells sense the mechanical properties of their surrounding environment and adapt accordingly to perform cellular functions such as adhesion, migration and differentiation. Integrin-mediated focal adhesions are major sites of mechanotransduction and their connection with the actomyosin network is crucial for mechanosensing as well as the generation and transmission of forces onto the substrate. Despite having emerged as major regulators of cell adhesion and migration, the contribution of microtubules to mechanotransduction still remains elusive. Here, we show that actomyosin-dependent mechanosensing of substrate rigidity controls microtubule acetylation, a tubulin post-translational modification, by promoting the recruitment of the alpha-tubulin acetyl transferase (αTAT1) to focal adhesions. Microtubule acetylation, in turn, promotes GEF-H1 mediated RhoA activation, actomyosin contractility and traction forces. Our results reveal a fundamental crosstalk between microtubules and actin in mechanotransduction, which contributes to mechanosensitive cell adhesion and migration.
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