乙酰胆碱
胆碱能的
细胞内
生物物理学
体内
生物
细胞生物学
神经科学
化学
乙酰胆碱受体
生物化学
受体
药理学
生物技术
作者
Miao Jing,Yuexuan Li,Jianzhi Zeng,Pengcheng Huang,Miguel Skirzewski,Ornela Kljakic,Wanling Peng,Tongrui Qian,Ke Tan,Jing Zou,Simon Trinh,Ruirui Wu,Shichen Zhang,Sunlei Pan,Samuel Andrew Hires,Min Xu,Hao‐Hong Li,Lisa M. Saksida,Vânia F. Prado,Timothy J. Bussey,Liangyi Chen,Heping Cheng
出处
期刊:Nature Methods
[Springer Nature]
日期:2020-09-28
卷期号:17 (11): 1139-1146
被引量:201
标识
DOI:10.1038/s41592-020-0953-2
摘要
The ability to directly measure acetylcholine (ACh) release is an essential step toward understanding its physiological function. Here we optimized the GRABACh (GPCR-activation-based ACh) sensor to achieve substantially improved sensitivity in ACh detection, as well as reduced downstream coupling to intracellular pathways. The improved version of the ACh sensor retains the subsecond response kinetics, physiologically relevant affinity and precise molecular specificity for ACh of its predecessor. Using this sensor, we revealed compartmental ACh signals in the olfactory center of transgenic flies in response to external stimuli including odor and body shock. Using fiber photometry recording and two-photon imaging, our ACh sensor also enabled sensitive detection of single-trial ACh dynamics in multiple brain regions in mice performing a variety of behaviors.
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