Molecularly imprinted Monolithic column-based SERS sensor for selective detection of cortisol in dog saliva

Molecularly imprinted monolithic column embedded with silver nanoparticles (MIMC@Ag) was synthesized by in-situ polymerization with template and porogen inside capillary tube followed by silver precursor reduction and template/porogen removal for realizing Raman detection of cortisol. Dense silver nanoparticles generated within the monolith makes this kind of column suitable for surface enhanced Raman scattering (SERS) detection, designated as SERS-MIMC. Scanning electron microscopy and BET profiler confirmed larger pore structure in the column after template removal. The corresponding increased mass transfer/binding rate, selective adsorption and adsorptive mechanism of the MIMC were well studied with a series of adsorption experiments. The minimum Raman detectable concentration of cortisol is 1 × 10-7 mol L-1 by using MIMC@Ag with a good linear relationship in the concentration range from 1 × 10-3 to 1 × 10-7 mol L-1. SERS sigmal of cortisol can be clearly distinguished from its analogs (estradiol, cholesterol and dexamethasone), proving the selective recognition of cortisol for SERS detection by MIMC@Ag. This ease-to-prepare SERS-MIMC sensor also shows good stability and reusability. The SERS-MIMC has been successfully applied for the easy, sensitive and selective detection of cortisol in dog saliva.