细胞内
细胞器
细胞生物学
线粒体
内质网
氧化应激
生物物理学
串扰
纳米医学
材料科学
纳米技术
纳米颗粒
生物
生物化学
光学
物理
作者
Vincenzo Migliaccio,Naym Blal,Micaela De Girolamo,Valentina Mastronardi,Federico Catalano,Ilaria Di Gregorio,Lillà Lionetti,Pier Paolo Pompa,Daniela Guarnieri
标识
DOI:10.1021/acsami.2c22375
摘要
The catalytic and antioxidant properties of platinum nanoparticles (PtNPs) make them promising candidates for several applications in nanomedicine. However, an open issue, still shared among most nanomaterials, is the understanding on how internalized PtNPs, which are confined within endo-lysosomal compartments, can exert their activities. To address this problem, here we study the protective effect of 5 nm PtNPs on a human hepatic (HepG2) cell line exposed to dichlorodiphenylethylene (DDE) as a model of oxidative stress. Our results indicate that PtNPs are very efficient to reduce DDE-induced damage in HepG2 cells, in an extent that depends on DDE dose. PtNPs can contrast the unbalance of mitochondrial dynamics induced by DDE and increase the expression of the SOD2 mitochondrial enzyme that recovers cells from oxidative stress. Interestingly, in cells treated with PtNPs─alone or in combination with DDE─mitochondria form contact sites with a rough endoplasmic reticulum and endo-lysosomes containing nanoparticles. These findings indicate that the protective capability of PtNPs, through their intrinsic antioxidant properties and modulating mitochondrial functionality, is mediated by an inter-organelle crosstalk. This study sheds new light about the protective action mechanisms of PtNPs and discloses a novel nano-biointeraction mechanism at the intracellular level, modulated by inter-organelle communication and signaling.
科研通智能强力驱动
Strongly Powered by AbleSci AI