橙皮苷
氧化应激
椎间盘
核心
细胞生物学
变性(医学)
细胞凋亡
NF-κB
生物
活性氧
信号转导
病理
医学
生物化学
解剖
替代医学
作者
Jian Zhu,Ruping Sun,Yan Chang,Kaiqiang Sun,Lu Gao,Bing Zheng,Jiangang Shi
出处
期刊:Cell Cycle
[Informa]
日期:2023-04-13
卷期号:22 (10): 1196-1214
被引量:5
标识
DOI:10.1080/15384101.2023.2200291
摘要
Intervertebral disc degeneration (IVDD), a widely known contributor to low back pain (LBP), has been proved to be a global health challenging conundrum. Hesperidin (hesperetin-7-O-rutinoside, HRD) is a flavanone glycoside that belongs to the subgroup of citrus flavonoids with therapeutic effect on various diseases due to its anti-inflammatory, antioxidant properties. However, the effect of HRD on IVDD remains elusive. The human nucleus pulposus tissues were harvested for isolating human nucleus pulposus (HNP) cells to verify the expression of Nrf2. The biological effect of HRD on HNP cells were assessed in vitro, and the in vivo therapeutic effects of HRD were assessed in mice. Firstly, we found that the expression of Nrf2 was decreased with the progression of degeneration in degenerated human nucleus pulposus tissue. Subsequently, we confirmed that HRD could mitigate oxidative stress-induced ferroptosis in nucleus pulposus cells via enhancing the expression of Nrf2 axis and suppressing the NF-κB pathway to protect intervertebral disc from degeneration in vitro. Finally, the therapeutic effects of HRD were confirmed in vivo. The current study proved for the first time that HRD may protect HNP cells from degeneration by suppressing ferroptosis in an oxidative stress-dependent via enhancing the expression of Nrf2 and suppressing the NF-κB pathway. The evidence will provide a possible basis for future targeted treatment for IVDD.
科研通智能强力驱动
Strongly Powered by AbleSci AI