植物化学
根茎
赫拉
化学
细胞凋亡
细胞毒性T细胞
皂甙
细胞毒性
糖苷
PI3K/AKT/mTOR通路
天然产物
立体化学
细胞培养
部分
流式细胞术
传统医学
IC50型
生物化学
生物
体外
分子生物学
病理
医学
替代医学
遗传学
作者
Tianyi Li,Yang Du,Minchang Wang,Ke Liu,Yang Liu,Yu Cao,Yuan‐Yuan Wang,Wenwen Chen,Xiao-Ying Qian,Pengcheng Qiu,Hai‐Feng Tang,Yang Lu
摘要
A phytochemical investigation of the steroidal saponins from the rhizomes of Paris polyohylla var. latifolia led to the discovery and characterization of three new spirostanol saponins, papolatiosides A–C (1–3), and nine known compounds (4–12). Their structures were established via extensive spectroscopic data analysis and chemical methods. Interestingly, compounds 1 and 2 possessed a fructosyl in their oligosaccharide moiety, which is rare in natural product and was firstly reported in family Melanthiaceae. The cytotoxicity of these saponins against several human cancer cell lines was evaluated by a CCK-8 experiment. As a result, compound 1 exhibited a significant cytotoxic effect on LN229, U251, Capan-2, HeLa, and HepG2 cancer cells with IC50 values of 4.18 ± 0.31, 3.85 ± 0.44, 3.26 ± 0.34, 3.30 ± 0.38 and 4.32 ± 0.51 μM, respectively. In addition, the result of flow cytometry analysis indicated that compound 1 could induce apoptosis of glioma cells LN229. The underlying mechanism was explored by network pharmacology and western bolt experiments, which indicated that compound 1 could induce glioma cells LN229 apoptosis by regulating the EGFR/PI3K/Akt/mTOR pathway.
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