Analytical and preparative chiral supercritical fluid chromatography resolutions using crown ether‐derived column

Abstract In this study, crown ether‐derived column Crownpak® CR‐I (+) was evaluated under SFC conditions using 12 primary amines, and the chromatographic results were compared against eight immobilized polysaccharide‐based columns. Crownpak® CR‐I (+) achieved a significantly higher success rate. It was found that the addition of 5% water to the modifier dramatically improved the peak shape for chiral separation of primary amines on Crownpak® CR‐I (+). The first reported preparative SFC separations on Crownpak® CR‐I (+) are shown, offering a new approach for the preparative resolution of primary amines. The case studies demonstrate that Crownpak® CR‐I (+) is a very useful column in the chiral separation of challenging compounds that contain a primary amine group in the pharmaceutical industry.