PI3K/AKT/mTOR通路
蛋白激酶B
细胞凋亡
信号转导
药理学
机制(生物学)
便秘
免疫印迹
LY294002型
医学
化学
癌症研究
内科学
生物化学
哲学
认识论
基因
作者
Hanyu Zhang,Fengyin Diao,Xuejin Fan,Sheng Ge,Xu Han,Luge Wei,Qingsheng Yin,Pengwei Zhuang,Mixia Zhang,Yanjun Zhang
标识
DOI:10.1016/j.jff.2023.105821
摘要
Nourishing Yin and Moistening Dryness Formula (NYMD) is a functional food known for its ability to nourish Yin and relieve constipation symptoms. However, the specific molecular mechanism by which it improves Yin-deficiency constipation (YC) is not well understood. This study used metabolomics, network pharmacology, and experimental validation to investigate the effect mechanism of NYMD on YC. Metabolomics analysis identified 34 YC-specific metabolites, while HPLC-Q-TOF/MS confirmed 33 major components of NYMD. Network pharmacology predicted that NYMD may regulate the PI3K/AKT signaling pathway in treating YC. Experimental validation through Western Blot and apoptosis assay confirmed that NYMD inhibits colon cell apoptosis through the PI3K/AKT signaling pathway, alleviating YC. Further confirmation was obtained by blocking the pathway using the PI3K inhibitor LY294002. These findings highlight the PI3K/AKT pathway as the primary mechanism of NYMD to improve YC. This study has significant implications for YC treatment, NYMD development, and its comprehensive utilization.
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