The biological activity, functionality, and emulsion stability of soybean meal hydrolysate–proanthocyanidin conjugates

The use of by-product hydrolysates as functional ingredients in food production is becoming more widespread. We hypothesized that the covalent binding of proanthocyanidin (PC) to soybean meal hydrolysates (SMHs) will improve the biological activity and function of the SMHs. Accordingly, we investigated the structure, antioxidant activity, and emulsion stability of SMHs after covalent conjugation with different concentrations of PC. An increase in PC addition resulted in the development of more high-molecular-weight SMHs–PC conjugates (40 kDa). The observed increase in the random coil content indicated that greater unfolding and disordered structure formation occurred with increasing PC addition. In addition, the fluorescence intensity and surface hydrophobicity of the SMHs increased, suggesting the presence of free amino acids, which likely contributed to the antioxidant activity and emulsifying properties of the SMHs. Addition of 3.0 mg/mL PC gave the SMHs–PC conjugates the highest antioxidant activity (ABTS+ and DPPH radical scavenging capacities of 89.08 ± 0.47 and 40.90 ± 1.53%, respectively) and emulsifying activity index (79.13 ± 2.80 m2/g), which may be attributed to protein unfolding and maximization of the polyphenol content when PC was covalently bound to the SMHs. Moreover, the SMHs–PC emulsion with 2.0 mg/mL PC showed the smallest particle size and highest viscosity, presenting promising potential as an emulsifier with high biological activity in food.