Microbial Allies in Plastic Degradation: Specific bacterial genera as universal plastic-degraders in various environments

Microbiological degradation of polymers offers a promising approach for mitigating environmental plastic pollution. This study (i) elucidated the diversity and structure of bacterial microbiomes from distinct environments (landfill soil, sewage sludge, and river water) characterized by specific physicochemical parameters, and (ii) utilized environment-derived microbial cultures enriched with microplastics (MPs) to investigate the degradation of polymers and identify culturable bacterial strains contributing to the plastisphere. We found that alpha diversity was notably higher in river water (∼20%) compared to landfill soil and sewage sludge. Dominant phyla included Pseudomonadota in sewage sludge (39.1%) and water (23.7%), while Actinomycetota prevailed in soil (38.5%). A multistage experiment, involving successive subcultures of environmental microbiomes exposed to polypropylene (PP), polyvinyl chloride (PVC), polycarbonate (PC), and polylactic acid (PLA), facilitated the assessment of MPs degradation processes. Analysis of carbonyl indices CIs and FTIR spectra revealed substantial structural changes in the treatment PVC-landfill soil, as well as in PLA- and PC-sludge enriched cultures. Further, using enriched cultures as a source of microorganisms, the study obtained 17 strains of plastic degraders from landfill soil, 14 from sewage sludge, and 6 from river water. Remarkably, similar bacterial genera were isolated across environmental microbiomes regardless of the MPs substrate used in enriched cultures. Among the 37 identified strains, Pseudomonadota predominated (64.86%) and were accompanied by Bacteroidota (16.22%), Actinomycetota (13.51%), and Bacillota (5.41%). This study highlights the complex relationship between microbiome diversity and the biodegradation efficiency of plastics, showing the potential for using microbial communities in the plastic pollution management.