Embelin isolated from Embelia ribes derived silver nanoparticles and its application in breast cancer nanomedicine

Breast cancer is one of the most predominant cancers in female claiming millions of lives every year. Our current research work proposes that the synthesis of silver nanoparticles using phytochemical constituent Embelin from Embelia ribes fruits provides significant result as a potent anticancer agent along with its probable mechanism of action depicted by molecular docking. UV–Visible spectroscopy was used to characterise Embelin-AgNPs, and the maximum absorbance was recorded at 374.5 nm. Particle size determination indicated monodispersity of nanoparticles; particle size recorded as 25–30 nm and zeta potential analysis well explained the stability of the nanoparticles. In vitro cytotoxicity study was done by MTT assay on MCF-7 cell line. Embelin-AgNPs induced apoptosis in MCF-7 cells as measured by the Annexin-V PI apoptosis assay. To predict the binding mode of ligand and receptor and probable mechanism of action in-silico study was done against ER alpha and HER2 receptors of breast cancer cells by molecular docking and it has revealed Embelin could act as potential modulator against ER positive and HER2 positive breast cancers.