诱导多能干细胞
去细胞化
细胞生物学
细胞外基质
胚胎干细胞
干细胞
基质凝胶
层粘连蛋白
组织工程
化学
维生素连接蛋白
胚芽层
生物
生物医学工程
细胞
生物化学
纤维连接蛋白
医学
基因
作者
Ting Wang,Tao Yu,Chang-Yen Tsai,Zhao-Yu Hong,Wen-Hui Chao,Yi-Shuo Su,Suresh Kumar Subbiah,Remya Rajan Renuka,Shih-Tien Hsu,Gwo-Jang Wu,Akon Higuchi
出处
期刊:Progress in Molecular Biology and Translational Science
日期:2023-01-01
卷期号:: 63-107
标识
DOI:10.1016/bs.pmbts.2023.02.008
摘要
Human pluripotent stem cells (human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs)) have unlimited proliferative potential, whereas adult stem cells such as bone marrow-derived stem cells and adipose-derived stem cells have problems with aging. When hPSCs are intended to be cultured on feeder-free or xeno-free conditions without utilizing mouse embryonic fibroblasts or human fibroblasts, they cannot be cultured on conventional tissue culture polystyrene dishes, as adult stem cells can be cultured but should be cultivated on material surfaces grafted or coated with (a) natural or recombinant extracellular matrix (ECM) proteins, (b) ECM protein-derived peptides and specific synthetic polymer surfaces in xeno-free and/or chemically defined conditions. This review describes current developing cell culture biomaterials for the proliferation of hPSCs while maintaining the pluripotency and differentiation potential of the cells into 3 germ layers. Biomaterials for the cultivation of hPSCs without utilizing a feeder layer are essential to decrease the risk of xenogenic molecules, which contributes to the potential clinical usage of hPSCs. ECM proteins such as human recombinant vitronectin, laminin-511 and laminin-521 have been utilized instead of Matrigel for the feeder-free cultivation of hPSCs. The following biomaterials are also discussed for hPSC cultivation: (a) decellularized ECM, (b) peptide-grafted biomaterials derived from ECM proteins, (c) recombinant E-cadherin-coated surface, (d) polysaccharide-immobilized surface, (e) synthetic polymer surfaces with and without bioactive sites, (f) thermoresponsive polymer surfaces with and without bioactive sites, and (g) synthetic microfibrous scaffolds.
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