제주도 자생 식물 혼합추출물의 in vitro 생리활성 평가

In this study moisturizing and anti-wrinkle effects of water extract from Jeju island plant mixture such as Portulaca oleracea, Opuntia ficusindica var. saboten, aloe vera, Ecklonia cava were investigated in vitro. Hyaluronic acid (HA) is a highly hydrophilic molecule known to be associated with skin moisture, wrinkle formation. Also, many studies have reported that collagen and elastin are a major factor in skin wrinkle formation. Therefore, we performed MTT assay, HA synthesis, hyaluronic acid synthase-2 (HAS-2) gene expression, collagen synthesis, hyaluronidase, collagenase and elastase inhibition assay for evaluation of biological activities. ELISA assay revealed that mixture increased HA and collagen production and RT-PCR also showed that mixture increased mRNA level of HAS-2 gene in HaCaT cells. And other results showed prevention of hyaluronidase, collagenase and elastase activation. These results indicate Jeju island plants mixture have moisturizing and anti-wrinkle effects. Therefore, Jeju island plants mixture can be used as a functional cosmetic.