结缔组织增生
细胞外基质
病理
CD8型
医学
淋巴结
免疫系统
腺癌
癌细胞
免疫组织化学
转移
癌症研究
癌症
基质
生物
内科学
免疫学
细胞生物学
作者
Eran Nizri,Shoshi Bar-David,Asaf Aizic,Neta Sternbach,Guy Lahat,Ido Wolf,Joseph Klausner
出处
期刊:Pancreas
[Openventio Publishers]
日期:2019-03-01
卷期号:48 (3): 367-373
被引量:16
标识
DOI:10.1097/mpa.0000000000001261
摘要
Pancreatic ductal adenocarcinoma (PDAC) is characterized by a peritumoral proliferation of fibroblasts and extracellular matrix production known as desmoplasia. We aimed to study desmoplasia in PDAC lymph node (LN) metastases.We evaluated LNs from 66 patients with PDAC and LN metastases. We used immunohistochemistry and real-time polymerase chain reaction to phenotype the desmoplastic response.Desmoplasia was identified in 57% of patients with LN metastases (Des+). Cancer-associated fibroblasts (CAFs) in Des+ expressed α-smooth muscle actin and collagen 11A1. The latter expression was present only in CAFs but not in LN stroma or in LN metastases without desmoplasia (Des-). Desmoplasia was associated with upregulation of transforming growth factor β messenger RNA. Whereas numbers of CD8+ in tumor vicinity were not different between Des+ and Des- patients (78 [standard deviation {SD}, 57] vs 92 [SD, 52], P = 0.48, respectively), the numbers of GATA-3+ cells, a marker of T-helper 2 immune response was significantly increased (3.7 [SD, 6.3] for Des+ vs 1.3 [SD, 2.7] for Des-, P < 0.05).Lymph node desmoplasia is associated with CAF pattern activation and Th2 infiltration. Therapeutic modulation of desmoplasia may be relevant in the metastatic phase and influence antitumor immune response.
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