DNA测序
鉴定(生物学)
挑剔的有机体
计算生物学
背景(考古学)
生物
基因组学
数据科学
基因组
计算机科学
遗传学
基因
生态学
古生物学
细菌
作者
Kenneth G. Frey,Kimberly A. Bishop‐Lilly
出处
期刊:Methods in Microbiology
日期:2015-01-01
卷期号:: 525-554
被引量:17
标识
DOI:10.1016/bs.mim.2015.06.004
摘要
Over the past decade, the field of genomics has seen such drastic improvements in sequencing chemistries that high-throughput sequencing, or next-generation sequencing (NGS), is being applied to generate data across many disciplines. NGS instruments are becoming less expensive, faster, and smaller, and therefore are being adopted in an increasing number of laboratories, including clinical laboratories. Thus far, clinical use of NGS has been mostly focused on the human genome, for purposes such as characterizing the molecular basis of cancer or for diagnosing and understanding the basis of rare genetic disorders. There are, however, an increasing number of examples whereby NGS is employed to discover novel pathogens, and these cases provide precedent for the use of NGS in microbial diagnostics. NGS has many advantages over traditional microbial diagnostic methods, such as unbiased rather than pathogen-specific protocols, ability to detect fastidious or non-culturable organisms, and ability to detect co-infections. One of the most impressive advantages of NGS is that it requires little or no prior knowledge of the pathogen, unlike many other diagnostic assays; therefore for pathogen discovery, NGS is very valuable. However, despite these advantages, there are challenges involved in implementing NGS for routine clinical microbiological diagnosis. We discuss these advantages and challenges in the context of recently described research studies.
科研通智能强力驱动
Strongly Powered by AbleSci AI