Actinobacteria are the antagonistic bacteria able to release antagonistic compounds which inhibits the growth of other microbial species. This study was carried out to identify biologically potential Actinobacteria from natural source. This study also confirms antimicrobial nature of the Actinobacteria. Twenty six marine water samples was collected from different time and points in the Bay of Bengal and isolated thirteen actinobacterial isolates using different culture medium. All actinobacterial isolates were identified by macroscopic and microscopic observation. The isolated actinobacteria were screened for their antimicrobial activity against UTI pathogens. The marine isolate Stretomycetes sp. identified from marine water were found to be more efficient in the production of secondary metabolites. This will pave the way for further investigations to study their potential importance in combating pathogenic bacteria.