Biological characterization of fowl intestinal bacteriophage

More than ten bacteriophage of E. coli were isolated from the soil and the dung of the fowl-run, then three of named bacteriophage A, C, D which lysis E. coli virulently were selected to investigate biological characterizations. The results showed that high activities were obtained after the phages incubated at 50deg C for 1 h or 60deg C for 30 min. The phages could be alive at the range of pH from 4 to 12, Ca~(2+) or Mg~(2+) added to the medium could stimulate the lysis of phages. However, the formation of the plaque could be inhibited obviously by adding sodium citrate to the medium.