Extraction of Peptidoglycan in Lactobacillus acidophilus Cell Wall

Peptidoglycan in Lactobacillus acidophilus cell wall was extracted and purified by ultrasonic wave method,lysozyme method and trichloroacetic method.The optimal conditions for ultrasonic wave were ultrasonic power 400 W,ultrasonic time 30 min,ultrasonic temperature 40℃,yield rate of peptidoglycan was 10.2%;the optimal conditions for lysozyme method were lysozyme 4 mg/mL,55℃,5 h,pH 7,yield rate of peptidoglycan was 10.0%;yield rate of peptidoglycan by trichloroacetic method was 15.8%.So trichloroacetic method was selected to extract peptidoglycan in Lactobacillus acidophilus cell wall.