The Nature of the Interaction of the P1 Recombinase Cre with the Recombining Site loxP

Cre-Lox重组 Cre重组酶 重组酶 位点特异性重组 重组 噬菌体 FLP-FRT重组 遗传学 生物 质粒 遗传重组 DNA 化学 大肠杆菌 基因 转基因 转基因小鼠
作者
R H Hoess,Ken Abremski,Nat Sternberg
出处
期刊:Cold Spring Harbor Symposia on Quantitative Biology [Cold Spring Harbor Laboratory]
卷期号:49: 761-768 被引量:57
标识
DOI:10.1101/sqb.1984.049.01.086
摘要

The experiments reported here begin to define the molecular nature of the Cre-loxP interaction. It is instructive to compare some of the features of this system with the two other well-characterized site-specific recombination systems of phage lambda and transposon Tn3. In terms of the DNA required for a functional recombining site, the Cre-loxP system appears to be the simplest of the three, requiring only 34 bp of sequence to bind the recombinase. In contrast, both lambda and Tn3 systems have multiple binding sites for their respective recombinases (Hsu et al. 1980; Grindley et al. 1982). It is believed that these additional binding sites, although not the sites of cleavage and strand exchange, are somehow important in bringing the DNA into a suitable configuration necessary for recombination (Better et al. 1982; Grindley et al. 1982; Pollock and Nash 1983). DNA conformation, i.e., whether the DNA substrate is supercoiled or linear, also appears to play a more important role in both of these systems, whereas the Cre-loxP system works efficiently, irrespective of the DNA conformation. All of the recombinases mentioned above create staggered cuts in the DNA during the process of strand exchange and form a covalent attachment to the DNA at the site of cleavage (Reed and Grindley 1981; Craig and Nash 1983b; R.H. Hoess and K. Abremski, in prep.). It should be pointed out that covalent attachment to the DNA is a general feature that these recombinases share with topoisomerases (Gellert 1981). The nature of the cuts made in the DNA is more similar for Int and Cre than for Tn3 resolvase.(ABSTRACT TRUNCATED AT 250 WORDS)

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