Preliminary analysis on the acute toxicity of multiple poisonous organic matters in measuring algae infrared radiation

Measuring algae infrared radiation was a method to analyze acute toxicity.This method was based on the principle that the sensitive algae's infrared radiation changes caused by poisonous matters could be measured.Sensitive algae was a special kind of algae which had four characteristics,including big temperature differences,quick response,many poisons,and low dose responding to acute toxicity when they were used as test materials in algae infrared radiation.In the test of response to poisonous organic matters in measuring algae infrared radiation,joint action of poisonous matters affected the sensitivity of sensitive algae directly.Hence,it was necessary that the sensitive algae's sensitivity to the concentration of multiple poisonous organic matters should be known first in joint toxicity test.The test of response of sensitive algae to combinations of five kinds of poisonous organic matters provided the foundation for designing concentration of multiple poisonous organic matters in order to keep sensitivity in joint toxicity test.In this experiment Selenastrum capricornutum was used as sensitive algae,and experimental drugs included C6H6、C7H8、C8H10、C6H5Cl and CHCl3.Results showed that the fact that the mix of multiple poisonous organic matters indeed led to changes of temperature differences,and it proved that joint action really existed in the test.And at all kinds of combinations of multiple poisonous organic matters,from unitary to quintuple(concentration of multiple poisonous organic matters from 100 mg·L-1 to 500 mg·L-1 in algae),Selenastrum capricornutum produced temperature response that could be measured.It indicated that the experiment could be performed in this concentration range,and when the concentration was from 100 mg·L-1 to 200 mg·L-1 is the best.