Molecular weight and monosaccharide composition of Flammulina velutipes polysaccharides of Sichuan,Zhejiang and Fujian were respectively analyzed by HPLC and GC.The result shows that these three kind of Flammulina velutipes all have two kind of polysaccharides of different molecular weight,high molecular weight in 1064661~1293488 u and low molecular weight in 19136~19829 u,polysacchrides of high molecular weight of Sichuan and Zhejiang Flammulina velutipes both have five monosaccharide,which were rhamnose,arabinose,mannose,glucose,galactose,and Fujian Flammulina velutipe has only 4 monosaccharide without rhamnose.