磷酸化
磷酸酶
线粒体
细胞生物学
线粒体通透性转换孔
生物
蛋白质磷酸化
蛋白质组
程序性细胞死亡
细胞内
激酶
氧化磷酸化
细胞
生物化学
蛋白激酶A
细胞凋亡
作者
Gang Lu,Haipeng Sun,Paavo Korge,Carla M. Koehler,James N. Weiss,Yibin Wang
出处
期刊:Elsevier eBooks
[Elsevier]
日期:2009-01-01
卷期号:: 255-273
被引量:20
标识
DOI:10.1016/s0076-6879(09)05014-9
摘要
Protein phosphorylation is a major form of posttranslational modification critical to cell signaling that also occurs in mitochondrial proteome. Yet, only very limited studies have been performed to characterize mitochondrial‐targeted protein kinases or phosphatases. Recently, we identified a novel member of PP2C family (PP2Cm) that is a resident mitochondrial protein phosphatase which plays an important role in normal development and cell survival. In this chapter, we will describe the methods applied in the identification of PP2Cm as a resident mitochondrial protein phosphatase based on sequence analysis and biochemical characterization. We will also provide experimental protocols used to establish the intracellular localization of PP2Cm, to achieve loss and gain function of PP2Cm in cultured cells and intact tissue, and to assess the impact of PP2Cm deficiency on cell death, mitochondria oxidative phosphorylation and permeability transition pore opening.
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