Electrochemical chiral sensor based on molecularly imprinted polymer for determination of (1S,2S)-pseudoephedrine in dosage forms and biological sample

In this study, simple, eco-friendly, highly sensitive, and selective molecularly imprinted polymer-based electrochemical chiral (MIEC) sensors were fabricated for the detection of Pseudoephedrine (PSDO) enantiomers. The sensitive and selective platform was prepared by in-situ electropolymerization of o-phenylenediamine (o-PD) on a glass carbon electrode in the presence of the template molecule, PSDO. The MIP modified sensor surface was characterized with Raman spectrometer, contact angle measurements, and scanning electron microscopy (SEM) to confirm successful preparation. Furthermore, cyclic voltammetry and electrochemical impedance spectroscopy were applied to test the electrochemical property of the sensor. Under the optimal conditions, the linear response of (1S,2S)-PSDO was obtained in the range of 1x10-15 − 1x10-14 M in the commercial human serum samples and effervescent tablets. Moreover, the high recovery data were obtained as 100.5 % and 100.4 % in pharmaceutical dosage forms and commercial human serum samples by the proposed (1S,2S)[email protected]/GCE sensor, respectively. The developed (1S,2S)[email protected]/GCE sensor has an excellent selectivity towards (1R,2R)-PSDO with an imprinting factor of 4.97.