Impact of static magnetic field exposure on <i>Stim1</i> and <i>Itpr3</i> expression in hepatic cells of obese mice.

Objectives: This study investigates the effects of 2 mT static magnetic field (SMF) exposure for 1 h on the expression of <i>Stim1</i> and <i>Itpr3</i> genes in hepatic cells of obese mice. By examining these critical regulators of calcium (Ca²⁺) signaling and cellular metabolism, the research aims to elucidate the role of SMF in modulating molecular pathways essential for Ca²⁺ homeostasis and metabolic regulation in the context of obesity. Materials and Methods: Liver samples were obtained from C57BL/6J mice and preserved in RNALater. The samples were divided into two main groups: the control group, which received a standard diet, and the obese group, which was exposed to a high-fat diet. Furthermore, the obese group was stratified based on the duration of SMF exposure, including intervals of 0, 2, 7, 14, and 21 days (1 h per day with an intensity of Bmax = 2 mT). Statistical tests were conducted with a significance level of <i>p</i> < 0.05. Results: The research findings highlighted a noteworthy increase in the relative expression of <i>Stim1</i> and <i>Itpr3</i> among obese mice exposed to SMF for 7 days (obe7) and those exposed for 14 days (obe14) in comparison to the obese group without SMF exposure. Both the obe7 and obe14 groups exhibited no significant difference in relative <i>Stim1</i> expression when compared to the non-obese group. However, in terms of <i>Itpr3</i> expression, the obe14 group did not show a significant difference from the non-obese mouse group. The results of the correlation analysis unveiled a substantial and robust correlation between the relative expression of <i>Stim1</i> and <i>Itpr3 </i>(<i>r</i> = 0.627, <i>p</i> < 0.001). Conclusion: These findings suggest a potential link between SMF exposure, the expression of Ca²⁺ regulatory genes, and the intricate pathways involved in obesity-related molecular responses.