生物
雌蕊
胚珠
分生组织
同源异型基因
MADS箱
突变体
基因
转录组
细胞生物学
拟南芥
植物
雄蕊
遗传学
胚胎
基因表达
花粉
作者
Chaoqun Shen,Yueya Zhang,Gang Li,Shi Jin,Duoxiang Wang,Wanwan Zhu,Xiujuan Yang,Ludovico Dreni,Matthew R. Tucker,Danting Zhang
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2023-09-21
卷期号:36 (1): 65-84
标识
DOI:10.1093/plcell/koad246
摘要
Abstract Temperature is a major factor that regulates plant growth and phenotypic diversity. To ensure reproductive success at a range of temperatures, plants must maintain developmental stability of their sexual organs when exposed to temperature fluctuations. However, the mechanisms integrating plant floral organ development and temperature responses are largely unknown. Here, we generated barley and rice loss-of-function mutants in the SEPALLATA-like MADS-box gene MADS8. The mutants in both species form multiple carpels that lack ovules at high ambient temperatures. Tissue-specific markers revealed that HvMADS8 is required to maintain floral meristem determinacy and ovule initiation at high temperatures, and transcriptome analyses confirmed that temperature-dependent differentially expressed genes in Hvmads8 mutants predominantly associate with floral organ and meristem regulation. HvMADS8 temperature-responsive activity relies on increased binding to promoters of downstream targets, as revealed by a cleavage under targets and tagmentation (CUT&Tag) analysis. We also demonstrate that HvMADS8 directly binds to 2 orthologs of D-class floral homeotic genes to activate their expression. Overall, our findings revealed a new, conserved role for MADS8 in maintaining pistil number and ovule initiation in cereal crops, extending the known function of plant MADS-box proteins in floral organ regulation.
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